Open AccessProtein Synthesis in Toluene‐Treated Escherichia coli Exclusive Synthesis of Membrane Proteins
Author(s) -
HALEGOUA Simon,
HIRASHIMA Akikazu,
SEKIZAWA Jun,
INOUYE Masayori
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10869.x
Subject(s) - escherichia coli , toluene , chemistry , protein biosynthesis , membrane protein , membrane , biochemistry , organic chemistry , gene
Toluene‐treated Escherichia coli K12 cells were shown to be able to synthesize proteins. Protein synthesis in cells treated with toluene in the presence of Mg 2+ and at 0 oC, was completely dependent upon the external addition of an energy source, ATP, and was sensitive to antibiotics which are known to inhibit protein synthesis. The toluene‐treated cells were shown to exclusively synthesize membrane proteins.