Open AccessPurification of Six Neurotoxins from the Venom of Dendroaspis viridis
Author(s) -
BECHIS Guy,
GRANIER Claude,
RIETSCHOTEN Jurphaas,
JOVER Emmanuel,
ROCHAT Hervé,
MIRANDA François
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10832.x
Subject(s) - elapidae , venom , edman degradation , naja , size exclusion chromatography , viperidae , snake venom , biochemistry , chemistry , biology , chromatography , peptide sequence , enzyme , gene
Six neurotoxins were purified from Dendroaspis viridis venom using gel filtration and equilibrium chromatography. The amino acid sequences of two of these neurotoxins (72 and 73 residues, live disulphide bridges) have been determined using almost exclusively automated Edman degradation. These two sequences are very similar: the only difference lies in the presence of one extra glycine at the C‐terminal end of one of them. There is a good homology with the sequences of toxins now isolated from other Elapidae and Hydrophidae venoms