Open AccessThe Primary Structure of a Non‐initiating Methionine‐Specific tRNA from Brewer's Yeast
Author(s) -
GRUHL Hartmut,
FELDMANN Horst
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10780.x
Subject(s) - transfer rna , nucleotide , yeast , ribonuclease , methionine , oligonucleotide , protein primary structure , biochemistry , ribonuclease t1 , chemistry , pancreatic ribonuclease , biology , rna , rnase p , peptide sequence , amino acid , dna , gene
tRNA 3 Met , one of the non‐initiating methionine‐specific tRNAs in brewer's yeast was purified from bulk tRNA labelled with [ 32 P]phosphate by two column chromatographic steps. The primary structure of this tRNA was determined by the usual fingerprinting technique. Analyses of the isolated nucleotides and oligonucleotides from digests with pancreatic and T 1 ribonucleases were in good agreement and stated that tRNA 3 Met consists of 76 nucleotide residues including 13 minor nucleotides. Overlaps from which the complete sequence could be deduced were derived from the analyses of 15 fragments obtained by partial digestion with T 1 ribonuclease.