Open AccessAmino‐Acid Sequence of a Coelenterate Toxin: Toxin II from Anemonia sulcata
Author(s) -
WUNDERER Gert,
FRITZ Hans,
WACHTER Elmar,
MACHLEIDT Werner
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10778.x
Subject(s) - toxin , marine toxin , biology , chemistry , sequence (biology) , biochemistry
Toxin II from Anemonia sulcata, the main component of the sea anemone venom, consists of 47 amino acid residues which are interconnected by three disulfide bridges. The S‐aminoethylated polypeptide was coupled to activated glass beads and sequenced to position 33 by automated solidphase Edman degradation. Blanks arising from anchor points and the rest of the sequence were determined from tryptic peptides of the [ 14 C]carboxymethylated toxin. Toxin II shows no significant homologies with other known sequences of neurotoxins or cardiotoxins. It might constitute a new class of polypeptide toxins.