Open AccessIndolmycin Inhibits Prokaryotic Tryptophanyl‐tRNA Ligase
Author(s) -
WERNER Rolf G.,
THORPE Lance F.,
REUTER Wolfgang,
NIERHAUS Knud H.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10758.x
Subject(s) - transfer rna , dna ligase , rna ligase , chemistry , biology , computational biology , biochemistry , rna , dna , gene
Indolmycin specifically prevents the formation of tryptophanyl‐tRNA in a prokaryotic system in vitro using Lscherichia coli enzymes. However, the drug has little effect in an eukaryotic system in vitro (rat liver enzymes). Analysis of the type of inhibition revealed that indolmycin competes with tryptophan as a pure competitive inhibitor of prokaryotic tryptophanyl‐tRNA ligase