Open AccessIsolation and Characterization of Rat‐Lens Messenger RNAs
Author(s) -
COHEN Louis H.,
SMITS Dimphy P. E. M.,
BLOEMENDAL Hans
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10722.x
Subject(s) - lens (geology) , messenger rna , heterologous , crystallin , biology , rna , microbiology and biotechnology , biochemistry , gene , paleontology
Messenger RNA has been isolated from rat lens tissue. The mRNA species which codes for the A 2 chain of α‐crystallin, revealed the same extremely high sedimentation value (14S) as the corresponding messenger from calf lens. However, it has been shown that in rat lens the 14‐S messenger preparation directs the synthesis of an additional α‐crystallin chain, designated as αX with. an. approximate molecular weight of 24000. For comparative purpose crystallin synthesis has also been studied as well in cultured rat lens cells as in the rat lens cell‐free system