Open AccessLocalization and Possible Role of an Adenosine Triphosphatase in Chlorobium thiosulfatophilum
Author(s) -
BURNS David D.,
MIDGLEY Melvin
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10695.x
Subject(s) - atpase , adenosine triphosphatase , biochemistry , chemistry , enzyme , photophosphorylation , in vivo , cytoplasm , adenosine triphosphate , cytoplasmic streaming , biophysics , biology , chloroplast , microbiology and biotechnology , gene
1 Evidence is presented that the ATPase activity detected in cell extracts of Chlorobium thiosulfatophilum is bound to the cytoplasmic membrane rather than to the chlorobium vesicles. 2 The activity of this ATPase is inhibited in vitro by various carbodiimides, phloridzin and sodium azide. 3 The apparent K m for ATP is ∼ 0.2 mM and the enzyme shows product inhibition by ADP. 4 Photophosphorylation, characterized in vivo, is inhibited by many of the compounds that in hibit the ATPase.