Open AccessA Novel Pathway for the Synthesis of Solanidine in the Isolated Chloroplast from Greening Potatoes
Author(s) -
RAMASWAMY N. Krishnan,
BEHERE Arun G.,
NAIR P. Madhusudanan
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10659.x
Subject(s) - chloroplast , formate , serine , biochemistry , glycine , chemistry , greening , formate dehydrogenase , biosynthesis , chlorophyll , hydroxymethyl , stereochemistry , enzyme , biology , amino acid , organic chemistry , catalysis , ecology , gene
Synthesis of chlorophyll always preceded the synthesis of solanine in cold‐stored potatoes in the presence of dim light (30–40 lux). Isolated chloroplasts from green peelings were able to fix C0 2 . The evidence obtained showed a direct reduction of CO 2 to formate, which, was the primary product of fixation. Apart from this the chloroplasts were capable of incorporating NaH 14 C03, [ 14 C]formate, [2‐ 14 C]glycine, [2‐ 14 C]pyruvate, [2‐ 14 C]acetate, [2‐ 14 C]mevalonate and [U‐ 14 C]serine into the sol1tnidine moiety of the alkaloid. The intermediates in the pathway between CO 2 and acetate have been identified as formate, glycine, serine and pyruvate. The localization of the enzymes involved in the reaction sequence viz. serine hydroxymethyl‐transferase, and pyruvic dehydrogenase complex, in the isolated chloroplast has been established.