Open AccessStructural Determination of Three Glycoasparagines Isolated from the Urine of a Patient with Aspartylglycosaminuria
Author(s) -
LUNDBLAD Arne,
MASSON Parvesh K.,
NORDÉN Nils E.,
SVENSSON Sigfrid,
ÖCKERMAN PerArne,
PALO Jorma
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10651.x
Subject(s) - yield (engineering) , chemistry , beta (programming language) , urine , stereochemistry , amino acid , chromatography , biochemistry , physics , computer science , thermodynamics , programming language
Three different glycoasparagines have been isolated from the urine of a patient with aspartylglycosaminuria and their structures determined using sugar, amino acid and methylation analysis, enzymic degradation and measurements of the optical rotations. The structures were 2‐acetamido‐1‐N‐(4′‐L‐aspartyl)‐2‐deoxy‐β‐ d ‐glucopyranosylamine(yield 135 mg/l) β‐ d ‐galactopyranosyl‐(1 → 4)‐2‐acetamido‐1‐N‐(4′‐L‐aspartyl)‐2‐deoxy‐β‐D‐glucopyranosylamine (yield 35 mg/l), and α‐D‐mannopyranosyl‐(1 → 6)‐β‐ d ‐mannopyranosyl‐(1 → 4)‐2‐deoxy‐β‐ d ‐glucopyranosyl‐(1 → 4)‐2‐acetamido‐1‐N‐(4′‐L‐aspartyl)‐2‐deoxy‐β‐ d ‐glucopyranosylamine (yield 30 mg/l). The first two compounds have previously been described, whereas the third compound is different from any of the glycoasparagines isolated before.