Open AccessPhotosynthetic Activities of Membrane Preparations of the Blue‐Green Alga Phormidium luridum
Author(s) -
BINDER Andres,
TELOR Elisha,
AVRON Mordhay
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10648.x
Subject(s) - photophosphorylation , photosystem i , photosynthesis , photosystem ii , chemistry , membrane , biophysics , biochemistry , photosystem , electron transport chain , photochemistry , biology , chloroplast , gene
Techniques have been developed for the isolation of whole spheroplaste and membrane preparations from the blue‐green alga Phormidium luridum which are active in photosystem‐II‐sensitized and photosystem‐I‐sensitized electron‐transport reactions and in photophosphorylation. Attention is called to the necessity of supplying magnesium and phosphate throughout the preparative and assay procedures for obtaining active membranes. Selective washing of a proper membrane preparation is shown to remove a new protein factor, termed protein factor 1, which restores the ability of the preparation to catalyze photosystem‐I‐sensitized reactions.