Open AccessPhosphorylation of Intracellular Fructose in Bacillus subtilis Mediated by Phospho enol pyruvate‐l‐Fructose Phosphotransferase
Author(s) -
DELOBBE André,
CHALUMEAU Hervé,
CLAVERIE JeanMichel,
GAY Philippe
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10573.x
Subject(s) - fructose , phosphotransferase , pep group translocation , biochemistry , intracellular , bacillus subtilis , phosphorylation , chemistry , fructose 1,6 bisphosphatase , biology , mutant , bacteria , genetics , gene
Intracellular fructose provided by the sorbitol pathway in Bacillus subtilis , can be phosphorylated by the phospho enol pyruvate‐1‐fructose phosphotransferase which is known to mediate a vectorial metabolism. The fate of this intracellular fructose was studied using mutants lacking either the fructose 1‐phosphate pathway or the fructose 6‐phosphate pathway. It was shown that the phospho enol pyruvate‐dependent phosphorylation needs a prior exit of the sugar into the medium, this exit being probably catalysed by a transport system. A low affinity intracellular phospho enol pyruvate phosphotransferase system was found, which seems to be devoid of a physiological role.