Open AccessDetermination of the Amino‐Acid Sequence of the Ribosomal Protein S8 of Escherichia coli
Author(s) -
STADLER Herbert,
WITTMANNLIEBOLD Brigitte
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10424.x
Subject(s) - biochemistry , valine , cyanogen bromide , leucine , edman degradation , amino acid , alanine , isoleucine , asparagine , chemistry , peptide sequence , ribosomal protein , methionine , biology , ribosome , rna , gene
The primary structure of protein S8 from the 30‐S ribosomal subunit of Escherichia coli was determined mainly by automatic Edman degradation using a modified Beckman protein sequenator and the solid‐phase sequenator of Laursen. The complete sequence, containing 109 amino acids, was derived by analysing peptides from tryptic, chymotryptic, thermolysin, staphylococcal protease and cyanogen bromide digestion of the protein. The amino acid composition was found to be (aspartic acid) 6 , (asparagine) 3 , (threonine) 5 , (serine)s, (glutamic acid) 7 , (glutamine) 6 , (proline) s , (glycine) 6 , (alanine) 11 , (valine) 9 , (methionine) 4 , (isoleucine) 7 , (leucine) 9 , (tyrosine) 3 , (phenylalanine) 3 , (lysine) 11 , (arginine) 8 , (cysteine) 1 . S8 is a basic protein and binds to the 16‐S RNA; knowledge of its sequence is necessary for a detailed study of its interaction with the ribosomal RNA.