Open AccessCell‐Cycle Dependence of Two Nuclear Histone Kinase Enzyme Activities
Author(s) -
HARDIE D. Graham,
MATTHEWS Harry R.,
BRADBURY E. Morton
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10422.x
Subject(s) - physarum polycephalum , histone , histone h1 , biochemistry , kinase , enzyme , cyclin dependent kinase 3 , cell cycle , chemistry , microbiology and biotechnology , biology , cell , protein kinase a , cyclin dependent kinase 2 , dna
Growth‐associated histone kinases have been extracted from Physarum polycephalum nuclei and resolved into two components by ion‐exchange chromatography. The two component activities have different substrate specificities and different times of appearance in the cell cycle. It is proposed that the enzyme(s) phosphorylate H1 histone in vivo in G2 phase, possibly sequentially in time at different sites in the H1 amino acid sequence.