Open AccessLipopolysaccharide from Yersinia pseudotuberculosis, Type 1B
Author(s) -
TOMSHICH Svetlana V.,
GORSHKOVA Raisa P.,
ELKIN Yury N.,
OVODOV Yury S.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10405.x
Subject(s) - yersinia pseudotuberculosis , mannose , periodate , chemistry , hydrolysis , polysaccharide , fucose , lipopolysaccharide , glucosamine , biochemistry , acid hydrolysis , lipid a , methylation , stereochemistry , galactose , biology , virulence , gene , endocrinology
Mild acid hydrolysis of lipopolysaccharide from Yersinia pseudotuberculosis, type 1B, resulted in the formation of an O‐specific polysaccharide. The repeating unit of this polysaccharide represented a pentasaccharide composed of residues of d ‐mannose, l ‐fucose, paratose (3,6‐dideoxy‐D‐glucose) and N‐acetyl‐ d ‐glucosamine. The data of methylation analysis, periodate oxidation, and partial hydrolysis demonstrated that the structure of the chemical repeating unit of the O‐specific chains seemed to be:where Par f is 3,6‐dideoxy‐ d ‐glucofuranose (paratose), Man p is mannopyranose, GIcNAc p is 2‐acetamido‐2‐deoxyglucopyranose, and Fuc p is fucopyranose.