Open AccessExistence, Localization and Regulation of the Biosynthesis of Aminoendopeptidase in Gram‐Negative Bacteria
Author(s) -
MURGIER Maryse,
PELISSIER Corinne,
LAZDUNSKI Andrée,
LAZDUNSKI Claude
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10368.x
Subject(s) - gram negative bacteria , escherichia coli , bacteria , gram , enterobacteriaceae , enzyme , pseudomonas , biosynthesis , antiserum , biology , microbiology and biotechnology , biochemistry , substrate (aquarium) , bacillus (shape) , escherichia , gene , genetics , antibody , ecology
An enzyme capable of hydrolyzing the substrate l ‐alanine p ‐nitroanilide has been found in 18 gram‐negative Enterobacteriaceae and Pseudomonas that we tested. This enzyme might be located near the cell surface. Contrastingly we have not detected this activity on colonies of the 8 grampositive Bacillus that we tested, which sugest a taxonomic value for this test. The aminoendopeptidases found in the various grain‐negative bacteria showed similar electrophoretic mobilities and immunological cross‐reactivity when tested against Escherichia coli anti‐aminoendopeptidase antiserum. The regulation of aminoendopeptidase biosynthesis by the endogenous level of inorganic phosphate that we previously demonstrated in E. coli has been found to exist in all gram‐negative organisms tested.