Open AccessThermal Melting Curves of tRNA Phe from Yeast Lacking Different Numbers of Nucleotides from the 3′‐End
Author(s) -
BELTCHEV Beltcho,
YANEVA Mariana,
STAYNOV Dontcho
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10329.x
Subject(s) - nucleotide , melting curve analysis , melting temperature , chemistry , yeast , thermal stability , crystallography , biochemistry , materials science , organic chemistry , real time polymerase chain reaction , gene , composite material
The thermal melting curves of tRNA Phe and tRNA Lys from yeast lacking different numbers of nucleotides from the 3′‐end were recorded. The removal of the first nucleotide had no effect on the melting profile. The stepwise removal of the following six nucleotides caused changes in the character of the derivative melting curves: they became rather broad and melting started at lower temperatures. These results indicate that the nucleotides at the 3′‐end contribute to the stability of the molecular structure of tRNAs.