Open AccessEnzymatic Synthesis of Two Fucose‐Containing Glycolipids with Fucosyltransferases of Human Serum
Author(s) -
PACUSZKA Tadeusz,
KOŚCIELAK Jerzy
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10328.x
Subject(s) - lactosylceramide , fucosyltransferase , glycolipid , fucose , fucosidase , chemistry , globotriaosylceramide , biochemistry , exoglycosidase , glycosphingolipid , substrate (aquarium) , enzyme , ceramide , lectin , glycoprotein , chromatography , glycan , biology , fabry disease , medicine , ecology , disease , pathology , apoptosis
Lacto‐ N ‐neotetraosylceramide incubated with human serum fucosyltransferase preparations gave rise to two fucoglycolipids. The faster migrating fucoglycolipid I on the basis of its thin‐layer chromatographic, mobility, susceptibility to α(1→2) fucosidase from Trichomonas foetus , radioimmunoprecipitation with Ulex europeus lectin and studies with O h (Bombay) sera was identified as H‐active glycolipid (H‐I). The most probable structure of fucoglycolipid II should be that with fucose linked α(1→3) to N‐acetylglucosamine. Lactosylceramide, ceramide trihexoside and globoside were not substrates for human serum fucosyltransferases. Lacto‐ N ‐neotetraosyl ceramide served as a fucose acceptor for all serum preparations tested while asialoganglioside was a substrate only when serum preparations containing H‐gene dependent α‐2‐ l ‐fucosyltransferase were used. With asialoganglioside only one radioactive reaction product was formed.