Open AccessTransglycosylic Reactions of Nucleotides of Deoxy Sugars
Author(s) -
ZEMEK Jiři.,
STRMEŇ Ján.,
KULČÁR Štefan.,
BAUER Štefan.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10298.x
Subject(s) - trehalose , hexose , chemistry , transferase , nucleotide , substrate (aquarium) , biochemistry , d glucose , biosynthesis , nucleotide sugar , yeast , enzyme , catalysis , stereochemistry , biology , ecology , gene
The biosynthesis of α, α′‐trehalose was investigated in a reaction catalyzed with yeast UDP‐Glc:glucose‐6‐phosphate 1‐glucosyl transferase. It was revealed that the hydroxyls at positions C‐2, C‐3, C‐4 and C‐6 are not essential for preserving its substrate properties in this reaction. The affinity of the enzyme and the rate of hexose incorporation into trehalose decreases in following sequence: UDP‐glucose, UDP‐2‐deoxy‐ d ‐glucose, UDP‐6‐deoxy‐ d ‐glucose, UDP‐4‐deoxy‐ d ‐glucose and UDP3‐deoxy‐ d ‐glucose.