Open AccessThe Binding of Tritiated Elongation‐Factors 1 and 2 to Ribosomes from Krebs II Mouse Ascites‐Tumor Cells
Author(s) -
NOLAN Robert D.,
GRASMUK Hans,
DREWS Jürgen.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10275.x
Subject(s) - ribosome , cycloheximide , diphtheria toxin , elongation factor , ricin , biochemistry , elongation , protein biosynthesis , chemistry , biology , toxin , rna , materials science , ultimate tensile strength , metallurgy , gene
The effect of a number of different antibiotics and toxins on the capacity of Krebs II mouse ascites ribosomes to bind 3 H‐labelled elongation factors (EF‐1 and EF‐2) has been examined. It was found that abrin and ricin inhibit the binding of EF‐2, while diphtheria toxin, sparsomycin, streptovitacin A, and cycloheximide had essentially no effect on its binding. Of the other compounds examined, sparsomycin was unique in its capacity, under some circumstances, to significantly affect the binding of aminoacyl‐tRNA and EF‐1 to ribosomes. Fusidic acid appears to nonspecifically enhance the binding of both EF‐1 and EF‐2.