Open AccessRat‐Liver Superoxide Dismutase
Author(s) -
REISS Uzi,
GERSHON David
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10266.x
Subject(s) - antigenicity , enzyme , superoxide dismutase , specific activity , cytoplasm , biochemistry , enzyme assay , chemistry , microbiology and biotechnology , biology , antibody , immunology
Cytoplasmic superoxide dismutase has been purified from livers of young (6 months) and old (27 months) rats. The enzyme purified from old animals shows an age‐related reduction in the specific activity, accumulation of antigenically cross‐reacting material and increased sensitivity to temperature. No differences were found in the molecular weight, electrophoretic mobility, antigenicity and K i between enzymes purified from young and old rats. This is the first demonstration of age‐related alterations in a purified form of a non‐metabolic enzyme, which can be related to reduced activity. The possible role of this reduced activity in agedependent deterioration of cellular functions is discussed.