Open AccessMethylation Analysis of Glucosaminitol and Glucosaminyl‐glucosaminitol Disaccharides
Author(s) -
HASE Sumihiro,
RIETSCHEL Ernst Th.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10211.x
Subject(s) - trimer , chemistry , hydrolysis , methylation , chromatography , acid hydrolysis , mass spectrometry , organic chemistry , biochemistry , dimer , gene
N ‐Acetylglucosaminitol, N ‐acetylglucosaminyl‐β 1′,4‐ N ‐acetylglucosaminitol (chitobiitol), the corresponding trimer (chitotriitol) and N ‐acetylglucosaminyl‐β 1′,6‐ N ‐acetylglucosaminitol were subjected to methylation analysis using combined gas‐liquid chromatography/mass spectrometry. The preparations were subjected to permethylation according to Hakomori, followed by acid hydrolysis. reduction and peracetylation. From N ‐acetylglucosaminitol and its 4‐0 and 6‐0 substituted derivatives, methylated N ‐acetylacetamido derivatives were obtained. The possible conditions for the formation of these products are discussed.