Open AccessOrganization of Vitellogenin Polysomes, Size of the mRNA and Polyadenylate Fragment
Author(s) -
JOST JeanPierre,
PEHLING Gundula
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10161.x
Subject(s) - polysome , vitellogenin , messenger rna , polyacrylamide gel electrophoresis , microbiology and biotechnology , biology , nucleotide , protein biosynthesis , biochemistry , gel electrophoresis , rna , chemistry , ribosome , enzyme , gene
A heavy polysome fraction containing vitellogenin mRNA was isolated from the liver of oestradiol‐treated chicks. As determined by urea – polyacrylamide gel electrophoresis, the molecular weight of vitellogenin mRNA is about 2.5 × 10 6 . The mRNA contains a polyadenylate segment of about 220 nucleotides at the 3′ end. The remaining 7000 nucleotides are sufficient to code for a polypeptide of M r about 270000. Combining ‘run off’ experiments of heavy polysomes in vitro together with radioimmunoprecipitation and polyacrylamide gel electrophoresis of the translation product, we concluded that vitellogenin mRNA is probably monocistronic and the 2.5 × 10 6 − M r mRNA codes for two polypeptides, M r 30000 and 240000. The largest polypeptide is, in our cellfree system and liver homogenate, readily cleaved into smaller peptides.