Open AccessN ‐Acetyl‐9‐ O ‐ l ‐lactylneuraminic Acid, a New Acylneuraminic Acid from Bovine Submandibular Gland
Author(s) -
SCHAUER Roland,
HAVERKAMP Johan,
WEMBER Margret,
VLIEGENTHART Johannes F. G.,
KAMERLING Johannis P.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10153.x
Subject(s) - chemistry , chromatography , periodate , acid hydrolysis , hydrolysis , mass spectrometry , biochemistry
The acylneuraminic acid fraction, obtained on mild acid hydrolysis of glycoproteins from bovine submandibular glands, contains approximately 2 % N‐acetyl‐9‐ O ‐ l ‐lactylneuraminic acid. The compound has been isolated and purified by ion‐exchange and cellulose column chromatography. The structure has been elucidated using thin‐layer chromatography, colorimetry, gas‐liquid chromatography/mass spectrometry, periodate oxidation and specific lactate dehydrogenases. An evaluation of the different analytical methods is given.