Open AccessRegulation of Cyanide‐Insensitive Respiration in Neurospora
Author(s) -
EDWARDS David L.,
ROSENBERG Emiko
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10150.x
Subject(s) - cyanide , neurospora crassa , neurospora , biology , respiration , mitochondrion , protein biosynthesis , biochemistry , respiratory chain , mitochondrial ribosome , cellular respiration , microbiology and biotechnology , mutant , ribosome , gene , chemistry , rna , botany , inorganic chemistry
1 Inhibition of either mitochondrial transcription or translation in Neurospora crassa results in the rapid production of the cyanide‐insensitive pathway of mitochondrial respiration. Protein synthesis an cytoplasmic ribosomes is required for the appearance of cyanide‐insensitive respiration in the culture. 2 Removal of the inhibition of transcription or translation results in a rapid return to cyanide‐sensitive respiration. Additional protein synthesis in the cytoplasm is required for the loss of cyanide‐insensitive respiratory activity while additional mitochondrial protein synthesis has no effect. 3 These studies indicate that a mitochondrial gene product is involved in a negative manner in the regulation of cyanide‐insensitive respiration. When the mitochondrial product is present, the pathway is not expressed, when the mitochondrial product is absent, the pathway is expressed. 4 Studies with forced heterokaryons formed from respiratory‐deficient mutants having cyanideinsensitive respiration and respiratory‐competent auxotrophs indicate that the site of action of the mitochondrial gene product is external to the mitochondrion.