Open AccessEffect of Triiodothyronine on Rat Liver Chromatin Protein Kinase
Author(s) -
KRUH Jacques,
TICHONICKY Lydie
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10103.x
Subject(s) - chromatin , triiodothyronine , histone , protein kinase a , enzyme , hormone , biology , medicine , endocrinology , cytosol , non histone protein , biochemistry , microbiology and biotechnology , chemistry , dna
1 Injection of triiodothyronine to rats stimulates protein kinase activity in liver chromatin non‐histone proteins. A significant increase was found after two daily injections. A 4‐fold increase was observed with the purified enzyme after eight daily injections of the hormone. No variations were observed in cytosol protein kinase activity. Electrophoretic pattern, effect of heat denaturation, effect of p‐hydroxymercuribenzoate seem to indicate that the enzyme present in treated rats is not identical to the enzyme in control animals, which suggests that thyroid hormone has induced nuclear protein kinase. Diiodothyronine, 3,3′,5′‐triiodothyronine have no effect on protein kinase. 2 Chromatin non‐histone proteins isolated from rats injected with triiodothyronine incorporated more 32 P when incubated with [γ‐ 32 P]ATP than the chromatin proteins from untreated rats. Thyroidectomy reduced the in vitro 32 P incorporation.It is suggested that some of the biological activity of thyroid hormone could be mediated through its effect on chromatin non‐histone proteins.