Open AccessCleavage Map of the Simian‐Virus‐40 Genome by the Restriction Endonuclease III of Haemophilus aegyptius
Author(s) -
YANG Robert CheAn,
VOORDE André,
FIERS Walter
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10002.x
Subject(s) - restriction enzyme , cleavage (geology) , haemophilus influenzae , dna , biology , hindiii , ecori , endonuclease , microbiology and biotechnology , simian , restriction fragment , restriction map , haemophilus , virus , pasteurellaceae , virology , plasmid , genetics , bacteria , paleontology , fracture (geology)
Enzymic digestion of Simian virus 40 (SV40) DNA with Haemophilus aegyptius restriction endonuclease Hae III results in 10 major and eight minor fragments. These were resolved by electrophoresis on graduated polyacrylamide slab gels. All fragments have been characterized with respect to the size relative to the Haemophilus influenzae Rd fragments (Hind). They were ordered on the SV40 DNA map by means of overlap analysis of the double cleavage products derived from sequential digestion of Hind fragments with Hae III endonuclease and Hae fragments with Hind II + III enzyme, as well as by other reciprocal cleavage experiments, including those involving Haemophilus parainfluenzae fragments. In this way the 18 Hae III cleavage sites and the 13 Hind sites have been localized on the circular SV40 DNA map.