Open AccessAnimal DNA‐Dependent RNA Polymerases
Author(s) -
KREBS Guy,
CHAMBON Pierre
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb09993.x
Subject(s) - oviduct , polymerase , rna , biology , rna polymerase , enzyme , microbiology and biotechnology , biochemistry , gene , endocrinology
Hen oviduct and liver class B RNA polymerases have been extensively purified and their molecular structure has been analysed. While only one enzyme B form (BIIb) was found in liver, three forms (BI, BIIa and BIIb) were resolved from oviduct. The molecular structures of the various class B RNA polymerase forms purified from hen oviduct and liver are identical to the corresponding forms previously purified from calf thymus and rat liver. At the present level of resolution the only difference lies in a slight difference in the charge of one subunit (SB2a) of enzyme form BIIa, when comparing the mammal and bird enzymes, It is unlikely that the absence of enzyme forms BI and Blla in purified hen liver RNA polymerase B could be related to limited and specific proteolysis during the purification, since co‐purification of oviduct and liver RNA polymerase B activities from a mixture of oviduct and liver nuclei does not affect the presence of either oviduct enzyme form BI or BIIa in the final purified mixture.