Open AccessThe Effect of 8‐Hydroxyquinoline on Enzyme Synthesis in the Fission Yeast Schizosaccharomyces pombe
Author(s) -
CREANOR James,
MAY John W.,
MITCHISON J. Murdoch
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb21027.x
Subject(s) - sucrase , schizosaccharomyces pombe , maltase , yeast , biochemistry , alkaline phosphatase , enzyme , chemistry , messenger rna , biology , saccharomyces cerevisiae , gene
The effect of 8‐hydroxyquinoline, a rapid inhibitor of RNA synthesis, was followed on the activity of a number of enzymes in cultures of the fission yeast Schizosaccharomyces pombe. Two types of effect were found. In the first the activity continued to rise for a period and then remained constant. This occurred with alkaline phosphatase, basal and derepressed acid phosphatase, hexokinase, and derepressed sucrase and maltase at low cell density. It is consistent with control being exercised by an unstable mRNA or by an unstable stimulator of translation. In the second the activity increásed above the control values for several hours. This occurred with basal sucrase and maltase, and suggests a stable mRNA and an unstable inhibitor of translation. The extent of ‘superproduction’ of sucrase varied with cell density and with growth medium and this may be due to differences in the degree of translational inhibition. The possibility of a stable mRNA has interesting implications for the control of enzyme synthesis through the cell cycle.