Open AccessPolyadenylated RNA from Vicia faba Meristematic Root Cells
Author(s) -
ESNAULT Robert,
TRAPY Gerard,
HUYSTEE Robert B.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb20978.x
Subject(s) - rna , nuclease , vicia faba , polysome , rna extraction , polyadenylation , hydrolysate , ribosomal rna , extraction (chemistry) , microbiology and biotechnology , sucrose , biochemistry , ribonuclease , chemistry , biology , chromatography , dna , ribosome , botany , hydrolysis , gene
After incubating root apices from two‐day‐old bean seedlings with [ 3 H]adenine the RNA was extracted from whole cells or polysomes, and the poly(A) sequences were isolated by nuclease digestion followed by poly(U)‐Sepharose chromatography. The alterations of the RNA molecules due to the various treatments were monitored by sucrose density gradients. It was found that sequential extraction first at pH 7.6 then at pH 9.0 did not result in a separation between RNA poor in poly(A) sequences and poly(A)‐rich RNA. Furthermore chromatography analysis of hydrolysates from nuclease‐resistant RNA extracted either at pH 7.6 or at pH 9.0 revealed that AMP constituted nearly 95% of the bases and that the poly(A) sequences, about 200 bases, were located at the 3′ terminus of the polyadenylated RNA. No size difference was found for the poly(A) segment between the pH‐7.6‐extracted RNA and that extracted at pH 9.0.