Open AccessBlocks in Elongation and Initiation of Protein Synthesis Induced by Interferon Treatment in Mouse L Cells
Author(s) -
CONTENT Jean,
NUDEL Uri,
ZILBERSTEIN Asher,
BERISSI Hanna,
REVEL Michel,
LEBLEU Bernard
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04106.x
Subject(s) - elongation , messenger rna , protein biosynthesis , ribosome , polypeptide chain , interferon , rna , elongation factor , transfer rna , translation (biology) , chemistry , microbiology and biotechnology , biochemistry , biology , gene , virology , enzyme , materials science , ultimate tensile strength , metallurgy
Synthesis of polypeptide chains coded by exogenous messenger RNAs is inhibited in cell‐free extracts from interferon‐treated mouse L cells, due to a “deficiency” in some specific tRNA species. A detailed analysis shows that polypeptide chain elongation is blocked and incomplete chains are formed. After a few minutes, however, initiation of new polypeptide chains is also blocked. Messenger RNA still binds to ribosomes but initiator Met‐tRNA fMet binding is inhibited. The block in initiation appears to be secondary to the block in elongation.