Open AccessImmunological Comparison of Basic Encephalitogen and Histone F2A1
Author(s) -
BUSTIN Michael,
TEITELBAUM Dvora,
WEBB Cynthia
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04105.x
Subject(s) - immunology , complement fixation test , stimulation , mixed lymphocyte reaction , histone , antibody , antigen , lymphocyte , delayed hypersensitivity , antiserum , immune system , radioimmunoassay , cellular immunity , biology , chemistry , biochemistry , endocrinology , t cell , serology , gene
The extent of immunological cross‐reaction between basic encephalitogen and histone F2A1 on both the humoral antibody level and on the cellular level has been established. The extent of humoral cross‐reaction was tested by direct complement fixation employing both anti‐histone F2A1 and antisera to basic encephalitogen, by inhibition of complement fixation, by radioimmunoassay and by passive cutaneous anaphylaxis. The data obtained failed to reveal immunological cross‐reaction between the proteins on the humoral antibody level. The extent of cross‐reaction at the cellular level was tested by the lymphocyte stimulation technique in rabbits and guinea pigs, by inhibition of lymphocyte stimulation and by delayed hypersensitivity skin reactions. A slight but significant cross‐reaction between the two proteins on the cellular level was detected by inhibition of lymphocyte stimulation and by the delayed hypersensitivity test. It is concluded that the immunological studies provide limited evidence that the two proteins share antigenic determinants.