Open AccessStudies on Bacteriophage M13 DNA
Author(s) -
HONDEL Cees A.,
SCHOENMAKERS John G. G.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04098.x
Subject(s) - restriction enzyme , cleavage (geology) , endonuclease , dna , bacteriophage , genome , restriction map , biology , microbiology and biotechnology , ecori , restriction fragment , genetics , gene , plasmid , escherichia coli , paleontology , fracture (geology)
A physical map of the bacteriophage M13 genome has been constructed on the basis of specific cleavage of M13 replicative form DNA by bacterial restriction endonucleases. The 13 fragments produced by the enzyme from Haemophilus aphirophilus (endonuclease R. Hap II) as well as the 10 fragments produced by the enzyme from Haemophilus aegyptius (endonuclease R. Hae III) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. In addition, the single site in M13 replicative form DNA cleaved by the restriction enzyme from Haemphilus influenzae Rd (endonuclease R. Hin dII) has been located more precisely. With this unique site as a reference point. the H. aphirophilus cleavage sites and the H. aegyptius cleavage sites have been loclaized on the map.