Open AccessIsolation and Characterization of Highly Purified Globin Messenger RNA from Anaemic‐Rabbit Spleen
Author(s) -
NOKIN Pierre,
BURNY Arsène,
CLEUTER Yvette,
HUEZ Georges,
MARBAIX Gérard,
CHANTRENNE Hubert
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04044.x
Subject(s) - reticulocyte , globin , rna , messenger rna , spleen , microbiology and biotechnology , biology , cell , biochemistry , chemistry , hemoglobin , immunology , gene
Highly purified globin messenger RNA has been isolated from the erythropoietic spleen of anaemic rabbits. This RNA, which sediments at 9‐S, directs the synthesis of α and β rabbit globin chains in a Krebs II ascites cell‐free system. Globin accounts for more than 90% of the cell‐free synthesized proteins. Spleen 9‐S RNA is, however, about 30% less effective on a weight basis than blood reticulocyte 9‐S RNA in directing globin synthesis in the cell‐free system. When analysed on polyacrylamide gels, spleen 9‐S RNA seems to be somewhat more heterogeneous in size than blood reticulocyte 9‐S RNA. Spleen globin mRNA contains a polyadenylic segment which is at least as long as the poly(A) tract of globin mRNA from blood reticulocytes.