Open AccessBiosynthesis of Nicotinamide Adenine Dinucleotide in Mitochondria
Author(s) -
GRUNICKE Hans,
KELLER Heinz J.,
PUSCHENDORF Bernd,
BENAGUID Abdelmoghit
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04039.x
Subject(s) - nad+ kinase , nicotinamide , nicotinamide adenine dinucleotide , nicotinamide mononucleotide , mitochondrion , biochemistry , chemistry , biosynthesis , quinolinic acid , niacinamide , azaserine , biology , enzyme , amino acid , tryptophan , glutamine
Isolated rat liver mitochondria incorporate [ 14 C]nicotinamide into NAD. Nicotinic acid and quinolinic acid are not utilized. The incorporation of nicotinamide into NAD is stimulated by ATP. After incubation of the mitochondria with [ 14 C]nicotinamide, labeled NMN can be isolated. No labeling of nicotinate mononucleotide or nicotinate adenine dinucleotide could be observed. Azaserine has no effect on the incorporation of nicotinamide into NAD. Labeled NAD is also obtained after addition of [ 14 C]ATP to the mitochondria. The incorporation of radioactivity from extramitochondrial [ 14 C]ATP into NAD is inhibited by carboxyatractyloside. On the basis of these results, it is concluded that the mitochondria incorporate nicotinamide by an intramitochondrial biosynthetic pathway via NMN.