Open AccessDissociation of Small‐Intestinal Sucrase. Isomaltase Complex into Enzymatically Active Subunits
Author(s) -
BRAUN Hersz,
COGOLI Augusto,
SEMENZA Giorgio
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04016.x
Subject(s) - sucrase , chemistry , enzyme , biochemistry , dissociation (chemistry) , small intestine , protein subunit , organic chemistry , gene
1 The sucrase. isomaltase complex from rabbit small intestine dissociated into its subunits upon reaction with citraconic anhydride. They can recombine after deacylation under mild acidic conditions. 2 When citraconylated, the subunits could be separated and isolated in a catalytically active form. 3 The previously reported procedure for separation of the subunits by alkaline treatment at pH 9.6 is apparently not due to contaminating degradative enzymes (possibly still present at undetectable levels in the isolated sucrase · isomaltase complex) but to the action of alkali.