Open AccessRNA‐Protein Interactions in the Ribosome
Author(s) -
SPIERER Pierre,
ZIMMERMANN Robert A.,
MACKIE George A.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb04014.x
Subject(s) - rna , ribosome , 5.8s ribosomal rna , nucleotide , signal recognition particle rna , biology , rna binding protein , biochemistry , protein subunit , microbiology and biotechnology , chemistry , computational biology , gene
Limited digestion of the Escherichia coli 50‐S subunit permits the isolation of two large fragments of the 23‐S RNA. One arises from the 5′ end of the 23‐S RNA and contains about 1200 nucleotides. The other arises from the 3′ end of the 23‐S RNA and contains about 2000 nucleotides. Each of the ten 503 proteins known to bind specifically and independently to the 23‐S RNA were tested for their ability to interact with these two RNA fragments. It was determined that the 5′ terminal segment contains the specific binding sites for proteins L4, L20 and L24, whereas the 3′ terminal segment contains the specific binding sites for proteins L1, L2, L3, L6, L13, L16 and L23.