Open AccessComputer Simulation Study of Hexokinase II from Ehrlich Ascites Cells
Author(s) -
GARFINKEL Lillian
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb03943.x
Subject(s) - hexokinase , ehrlich ascites , mechanism (biology) , enzyme , mitochondrion , chemistry , ternary complex , biochemistry , biophysics , glycolysis , biology , physics , tumor cells , cancer research , quantum mechanics
A study of the mechanism of hexokinase II from ascites cells and the effects of its binding to mitochondrial membranes has been carried out by computer simulation. This is based on experimental data of Kosow and Rose and of Gumaa and McLean, and the theoretical methods of Cleland. For the soluble enzyme the mechanism is random with ternary product‐inhibition complexes; when bound to mitochondria, the mechanism becomes ordered‐on, random‐off, as the binding of ATP to the free enzymes becomes negligibly slow. The requirements of experimental data for mechanistic studies are discussed.