Open AccessStudies on Energy‐Linked Reactions: Isolation and Properties of Mitochondrial Venturicidin‐Resistant Mutants of Saccharomyces cerevisiae
Author(s) -
GRIFFITHS David E.,
HOUGHTON Raymond L.,
LANCASHIRE William E.,
MEADOWS Patricia A.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb03939.x
Subject(s) - saccharomyces cerevisiae , isolation (microbiology) , mutant , mitochondrial dna , genetics , chemistry , biology , microbiology and biotechnology , yeast , gene
Venturicidin is a specific inhibitor of aerobic growth of yeast and has no effect on fermentative growth, a result which is consistent with its known mode of action on mitochondrial oxidative phosphorylation. Venturicidin‐resistant mutants of Saccharomyces cerevisiae have been isolated and form two general classes: class 1, nuclear mutants which are resistant to a variety of mitochondrial inhibitors and uncouplers, and class 2, mitochondrial mutants of phenotype VEN R OLY R and VEN R TET R in vivo. VEN R OLY R mutants show a high degree of resistance to venturicidin and oligomycin at the whole cell and mitochondrial ATPase level but, in contrast, no resistance at the mitochondrial level is observed with VEN R TET R mutants. Venturicidin resistance/sensitivity can be correlated with two binding sites on mitochondrial ATPase, one of which is common to the oligomycin binding site and the other is common to the triethyl tin binding site. Biochemical genetic studies indicate that two mitochondrial genes specify venturicidin resistance/sensitivity and that the mitochondrial gene products are components of the mitochondrial ATPase complex.