Open AccessA Urinary Pentasaccharide in Bovine Mannosidosis
Author(s) -
LUNDBLAD Arne,
NILSSON Bo,
NORDÉN Nils E.,
SVENSSON Sigfrid,
ÖCKERMAN PerArne,
JOLLY Robert D.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02488.x
Subject(s) - oligosaccharide , chemistry , chromatography , yield (engineering) , mannose , column chromatography , urine , mass spectrometry , paper chromatography , enzyme , nuclear magnetic resonance spectroscopy , biochemistry , stereochemistry , materials science , metallurgy
Abnormally high amounts of low molecular weight mannose‐rich carbohydrate material were found in the urine of an Angus calf with mannosidosis. At least five oligosaccharide fractions were detected by paper chromatography. The most abundant compound was purified by gel chromatography, zone electrophoresis, and two consecutive preparative paper chromatographic steps. The yield was 10 mg/liter of urine. From structural studies including nuclear magnetic resonance spectroscopy, optical rotation, sugar analysis, methylation analysis, and partial enzymatic degradation the following structure was deduced: α‐ d ‐Man p ‐(1→6)‐β‐ d ‐Man p ‐(1→4)‐β‐ d ‐GlcNAc p ‐(1→4)‐β‐ d ‐GlcNAc p ‐(1→4)‐ d ‐GlcNAc. This oligosaccharide is distinct from all the oligosaccharides previously described which are excreted by patients with mannosidosis.