Open AccessStudies Concerning the Mechanism by which Translational‐Control RNA Regulates Protein Synthesis in Embryonic Muscle
Author(s) -
HEYWOOD Stuart M.,
KENNEDY Doris S.,
BESTER André J.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02409.x
Subject(s) - messenger rna , translation (biology) , protein biosynthesis , translational frameshift , myosin , rnase p , messenger rnp , biology , microbiology and biotechnology , p bodies , rna , chemistry , biochemistry , gene
Muscle translational‐control RNA (tcRNA) has been separated into two classes, polysomal and messenger ribonuclear protein (mRNA · protein), which have different sizes as determined by acrylamide gel electrophoresis. While normally translation of mRNA · protein mRNA is inhibited by tcRNA derived from the same mRNA · proteins, this inhibition does not occur if the messenger is previously de‐adenylated. This suggests that the poly(A) segment of mRNA is required for the tcRNA activity. Utilizing different mRNA · protein fractions from muscle, myosin mRNA · protein and small mRNA · proteins (<30 S), we have been able to demonstrate that a degree of specificity exists in the interaction of tcRNA and mRNA derived from the same mRNA · proteins. This is illustrated by the facts that (a) each tcRNA only inhibits the translation of its respective mRNA and (b) the highest percentage of structural change occurs when each tcRNA is hybridized to its respective mRNA as measured by its resistance to T 1 and T 2 RNase.