Open AccessAmino‐Acid Sequence of the Cyanogen‐Bromide Fragment from Human Plasminogen that Forms the Linkage between the Plasmin Chains
Author(s) -
WIMAN Björn,
WALLÉN Per
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02403.x
Subject(s) - cyanogen bromide , plasmin , chemistry , peptide sequence , fragment (logic) , stereochemistry , sequence (biology) , amino acid , biochemistry , enzyme , gene , computer science , programming language
The complete amino acid sequence of a cyanogen bromide fragment (122 residues) obtained from plasminogen is described. This fragment forms the overlap between heavy (A) and light (B) chains of human plasmin. The particular arginyl‐valyl bond cleaved in the second step of the activation process is shown to be Arg 98 ‐Val 99 in this fragment. This site is not very similar to the one in the NH 2 ‐terminal part of the molecule (Arg 68 ‐Met 69 ). Remarkable homologies with the ‘triple loops’ (‘kringle structures’) found in the non‐thrombin part of prothrombin are demonstrated. Homologies within the plasmin heavy (A) chain are also demonstrated, suggesting that gene duplication has occurred during evolution of this chain.