Open AccessCo‐fractionation of an Endonuclease Activity during the Purification of DNA Polymerase‐α from Regenerating Rat Liver
Author(s) -
MECHALI Marcel,
RECONDO AnneMarie
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02393.x
Subject(s) - dna polymerase , endonuclease , polymerase , dna clamp , dna polymerase ii , microbiology and biotechnology , dna , biochemistry , dna polymerase i , fractionation , biology , chemistry , polymerase chain reaction , chromatography , reverse transcriptase , gene
The presence of endonuclease activity associated with DNA polymerase was detected during the purification of high‐molecular‐weight DNA polymerase‐α from regenerating rat liver by the use of a highly sensitive test. This endonuclease activity co‐fractionated with the DNA polymerase in a great variety of purification procedures involving ion‐exchange chromatographies or molecular weight fractionation, but was further completely separated from DNA polymerase activity by using affinity chromatography on DNA‐cellulose. The endonuclease acted on native or denatured DNA by introducing single‐strand nicks in the DNA molecules; its enzymatic properties indicate that it could act in polymerisation conditions in vitro .