Open AccessStudies on Norepinephrine‐Induced Efflux of Free Fatty Acid from Hamster Brown‐Adipose‐Tissue Cells
Author(s) -
BIEBER Loran L.,
PETTERSSON Bertil,
LINDBERG Olov
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02384.x
Subject(s) - lipolysis , adipose tissue , hamster , norepinephrine , medicine , endocrinology , fatty acid , extracellular , chemistry , brown adipose tissue , extracellular fluid , biology , biochemistry , dopamine
Cells were isolated from brown adipose tissue of warm‐adapted hamsters and the fate of free fatty acids released during norepinephrine‐induced lipolysis was investigated. The isolated resting cells contain between 100–400 nmoles cell‐associated free fatty acids per 10 6 cells; most preparations contained about 200 nmoles/10 6 cells. During norepinephrine‐stimulated lipolysis, the level of cell‐associated free fatty acids remains constant or decreases gradually, but does not increase, while the concentration of extracellular fatty acids increases linearly. The rate of norepinephrine‐stimulated efflux of free fatty acids was 40 ± 20 nmol x min ‐1 x 10 6 cells ‐1 ( n = 11) at 37°C. The data strongly indicate that brown adipose tissue can supply free fatty acids to the circulatory system in hamster.