Open AccessMapping of Cleavage Sites for Restriction Endonucleases in λdv Plasmids
Author(s) -
STREECK Rolf E.,
HOBOM Gerd
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02335.x
Subject(s) - ecori , plasmid , restriction enzyme , cleavage (geology) , microbiology and biotechnology , escherichia coli , bacillus subtilis , dna , restriction map , biology , restriction fragment , gel electrophoresis , restriction site , bacteriophage , genetics , gene , bacteria , paleontology , fracture (geology)
Restriction nucleases from Escherichia coli carrying the resistance transfer factor RI, from Hemophilus influenzae, Hemophilus parainfluenzae , and Bacillus subtilis were used for the generation of specific DNA fragments from λdv plasmids. Cleavage maps were established for various plasmids containing different but overlapping parts of bacteriophage λ DNA by analysis of digestion patterns obtained in polyacrylamide gel electrophoresis. The correlation between the plasmid cleavage maps and the genetic map of λ was based upon the location of the EcoRI cleavage site at 81.0% of λ fractional length and the position of promoters p L and p R on the plasmid DNA fragments.