Open AccessHydroxylation of Testosterone at Carbons 1, 2, 6, 7, 15 and 16 by the Hepatic Microsomal Fraction from Adult Female C57BL/6J Mice
Author(s) -
FORD Henry C.,
WHEELER Rachel,
ENGEL Lewis L.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02271.x
Subject(s) - microsome , chemistry , fraction (chemistry) , hydroxylation , testosterone (patch) , chromatography , metabolism , medicine , endocrinology , biochemistry , enzyme , biology
The metabolism of a mixture of [4‐ 14 C]‐ and [7β‐ 2 H]testosterone by the hepatic microsomal fraction from adult female C57BL/6J mice has been investigated. The following metabolites were identified by their mass spectra and by their retention times on gas chromatography on one or two phases: 1ζ‐, 2β‐, 6α‐, 6β‐, 7α‐, 15α‐, 15β‐, 16α‐ and 16β‐hydroxytestosterone; 6α‐, 6β‐ and 7α‐hydroxy‐4‐androstene‐3,17‐dione; and 4‐androstene‐3,17‐dione. A compound tentatively identified as 6‐ or 7‐oxotestosterone was also isolated. 17β‐Hydroxy‐4,6‐androstadien‐3‐one, 17β‐hydroxy‐1,4‐androstadien‐3‐one and 4,6‐androstadiene‐3,17‐dione were identified but are considered to arise non‐enzymatically from 7α‐hydroxytestosterone, 1ζ‐hydroxytestosterone and 7α‐hydroxy‐4‐androstene‐3,17‐dione, respectively.