Open AccessStabilization of Promoter Complexes with a Single Ribonucleoside Triphosphate
Author(s) -
NÜSSLEIN Christiane,
SCHALLER Heinz
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02263.x
Subject(s) - gtp' , ribonucleoside , dna , chemistry , biochemistry , pyrimidine , enzyme , rna polymerase , microbiology and biotechnology , stereochemistry , rna , biology , gene
Under specific binding conditions RNA polymerase forms complexes at several sites of the replicative form DNA of bacteriophage fd. One of these complexes becomes stable to both high salt and low temperature after incubation with GTP. None of the complexes is stabilized by ATP. The stabilization by GTP results from the synthesis of an oligo(G) chain, which is bound in the complex. Size and pyrimidine fingerprints of the DNA segment protected by the enzyme against digestion with DNase are not changed upon initiation of oligo(G) synthesis. This result indicates that binding site and initiation site are identical parts of a promoter region.