Open AccessSynthesis of Apamin, a Neurotoxic Peptide from Bee Venom
Author(s) -
RIETSCHOTEN Jurphaas,
GRANIER Claude,
ROCHAT Hervé,
MIRANDA Francois,
LISSITZKY Serge
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02204.x
Subject(s) - apamin , bee venom , chemistry , peptide , chromatography , peptide sequence , venom , biochemistry , biology , organic chemistry , calcium , zoology , gene
The apamin sequence has been synthesized by the solid‐phase procedure. The synthetic peptide showed the same physicochemical and chemical properties as natural apamin in the following tests: paper electrophoresis, amino acid analyses after acid and enzymatic hydrolyses, sequence analysis, electrophoreses after tryptic and chymotryptic digestions. Synthetic apamin was as active as natural apamin in the neurotoxic assay in mice (LD 50 , after subcutaneous injection, for the 20‐g mouse: 58 μg).