Open AccessProtein Kinase of Bacteriophage T7
Author(s) -
PAI ShouHsiung,
PONTA Helmut,
RAHMSDORF HansJobst,
HIRSCHKAUFFMANN Monica,
HERRLICH Peter,
SCHWEIGER Manfred
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02163.x
Subject(s) - escherichia coli , affinity chromatography , bacteriophage , lysozyme , enzyme , biochemistry , chemistry , ribosomal protein , sepharose , electrophoresis , protein purification , microbiology and biotechnology , chromatography , biology , ribosome , rna , gene
A protein kinase, ATP: protein phosphotransferase (EC 2.7.1.37) was detected in Escherichia coli after infection with bacteriophage T7. The enzyme was purified from the ribosomal wash fraction by conventional methods, affinity chromatography on Cibacron blue and on lysozyme coupled to Sepharose, and by cellogel electrophoresis. An approximately 5000‐fold purification was achieved.