Open AccessStudies on Phenylalanine‐Specific Transfer Ribonucleic Acid from Chick Embryos
Author(s) -
WITTIG Burghardt,
REUTER Stephanie,
GOTTSCHLING Hubert
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03856.x
Subject(s) - phenylalanine , embryo , transfer rna , hyperchromicity , tryptophan , fluorescence , chemistry , biochemistry , tyrosine , amino acid , rna , chromatography , stereochemistry , biology , dna , physics , quantum mechanics , gene , microbiology and biotechnology
The phenylalanine‐specific tRNA Phe from 13‐day‐old chick embryo muscle was purified. Its fluorescent and hydrophobic Y + base was excised by mild acid treatment and characterized chromatographically and by its ultraviolet absorption and fluorescent spectra. The compound obtained after removal of the Y + base from chick embryo tRNA Phe was characterized.1 Chick embryo tRNA Phe looses by the removal of the Y + base its phenylalanine acceptor capacity. 2 In this state it has a reduced sedimentation coefficient of 2.8 S, a low T m value (22°C), only rudimentary hyperchromicity, but unchanged electrophoretic properties. 3 The compound obtained after removal of the Y + base from chick embryo tRNA Phe regains normal melting behaviour and phenylalanine acceptor capacity when exposed to special renaturation conditions. 4 The K m , of the renatured form is 0.25 × 10 −6 M and V = 220 pmol × min −1 × ml −1 , as compared to K m , = 0.11 × 10 −6 M and V = 370 pmol × min −1 × ml −1 for the native chick embryo tRNA Phe .