Open AccessNitrate Reductase as a Product‐Inducible Enzyme
Author(s) -
KAPLAN Drora,
ROTHBEJERANO Nurit,
LIPS Herman
Publication year - 1974
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1974.tb03844.x
Subject(s) - nitrite , nitrate reductase , nitrate , nitrite reductase , chemistry , enzyme , substrate (aquarium) , biochemistry , enzyme inducer , inducer , cofactor , biology , organic chemistry , ecology , gene
Nitrite is a very effective inducer of nitrate reductase. Nitrate induces the enzyme in darkness only upon addition of suitable sources of reducing equivalent which permit the formation of nitrite; nitrite itself brings about induction of the enzyme in darkness without supplementation of exogenous reducing cofactors. Inhibition of constitutive nitrate reductase by tungstate prevents induction of the enzyme by nitrate but not by nitrite. Since nitrate induces the enzyme only under conditions allowing production of nitrite while the induction capacity of nitrite is free of this constraint, nitrate reductase be considered a product‐inducible rather than a substrate‐inducible enzyme.